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recombinant human glial cell line-derived neurotrophic factor  (PeproTech)


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    Structured Review

    PeproTech recombinant human glial cell line-derived neurotrophic factor
    Recombinant Human Glial Cell Line Derived Neurotrophic Factor, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+recombinant+glial+cell+line%E2%80%93derived+neurotrophic+factor/recombinant+human+gdnf/pmc12202018-70-53-55
    Average 90 stars, based on 1 article reviews
    recombinant human glial cell line-derived neurotrophic factor - by Bioz Stars, 2026-09
    90/100 stars

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    Incubation:

    Article Title: Inflammatory cytokines disrupt astrocyte exosomal HepaCAM-mediated protection against neuronal excitotoxicity in the SOD1G93A ALS model.
    Article Snippet: The MN pellets were gently resuspended with neuron plating medium containing DMEM, 10% of FBS (Sigma- Aldrich), and 1% Pen- Strep and plated on PDL + LN coated coverslips at 200,000 cells per well. .. MNs were incubated a 37°C for 12 hours, washed twice with warm neurobasal medium (Invitrogen), and cultured in 1 ml of MN medium: neurobasal medium supplemented with 2% B- 27 (Thermo Fisher Scientific), 1% of 100× GlutaMAX, hydrocortisone (0.2 μg/ml; Sigma- Aldrich), insulin (2.5 μg/ml; SigmaAldrich), human recombinant NT3 (10 ng/ml; ProspecBio), human recombinant glial cell line–derived neurotrophic factor (GDNF, 10 ng/ml; Peprotech), human recombinant brain- derived neurotrophic factor (BDNF, 10 ng/ml; R&D Systems), and human recombinant ciliary neurotrophic factor (25 ng/ml; Novus Biologicals). ..

    Cell Culture:

    Article Title: Inflammatory cytokines disrupt astrocyte exosomal HepaCAM-mediated protection against neuronal excitotoxicity in the SOD1G93A ALS model.
    Article Snippet: The MN pellets were gently resuspended with neuron plating medium containing DMEM, 10% of FBS (Sigma- Aldrich), and 1% Pen- Strep and plated on PDL + LN coated coverslips at 200,000 cells per well. .. MNs were incubated a 37°C for 12 hours, washed twice with warm neurobasal medium (Invitrogen), and cultured in 1 ml of MN medium: neurobasal medium supplemented with 2% B- 27 (Thermo Fisher Scientific), 1% of 100× GlutaMAX, hydrocortisone (0.2 μg/ml; Sigma- Aldrich), insulin (2.5 μg/ml; SigmaAldrich), human recombinant NT3 (10 ng/ml; ProspecBio), human recombinant glial cell line–derived neurotrophic factor (GDNF, 10 ng/ml; Peprotech), human recombinant brain- derived neurotrophic factor (BDNF, 10 ng/ml; R&D Systems), and human recombinant ciliary neurotrophic factor (25 ng/ml; Novus Biologicals). ..

    Recombinant:

    Article Title: Inflammatory cytokines disrupt astrocyte exosomal HepaCAM-mediated protection against neuronal excitotoxicity in the SOD1G93A ALS model.
    Article Snippet: The MN pellets were gently resuspended with neuron plating medium containing DMEM, 10% of FBS (Sigma- Aldrich), and 1% Pen- Strep and plated on PDL + LN coated coverslips at 200,000 cells per well. .. MNs were incubated a 37°C for 12 hours, washed twice with warm neurobasal medium (Invitrogen), and cultured in 1 ml of MN medium: neurobasal medium supplemented with 2% B- 27 (Thermo Fisher Scientific), 1% of 100× GlutaMAX, hydrocortisone (0.2 μg/ml; Sigma- Aldrich), insulin (2.5 μg/ml; SigmaAldrich), human recombinant NT3 (10 ng/ml; ProspecBio), human recombinant glial cell line–derived neurotrophic factor (GDNF, 10 ng/ml; Peprotech), human recombinant brain- derived neurotrophic factor (BDNF, 10 ng/ml; R&D Systems), and human recombinant ciliary neurotrophic factor (25 ng/ml; Novus Biologicals). ..

    Article Title: SARS-CoV and SARS-CoV-2 display limited neuronal infection and lack the ability to transmit within synaptically connected axons in stem cell–derived human neurons
    Article Snippet: NPCs were then maintained in STEMdiffTM Neural Progenitor Medium (STEMCELL Technologies) supplemented with 1 × STEMdiff neural progenitor supplement A (STEMCELL Technologies) and 1 × STEMdiff neural progenitor supplement B (STEMCELL Technologies) at 37 °C and 5% CO 2 . .. Neural differentiation was initiated by replacing 50% v/v of the culture medium every 2–3 days with BrainPhysTM neural medium (STEMCELL Technologies) supplemented with 2% v/v NeurocultTM SM1 Neuronal Supplement (STEMCELL Technologies), 1% v/v N2 Supplement-A (STEMCELL Technologies), 20 ng/mL human recombinant brain-derived neurotrophic factor, 20 ng/mL human recombinant glial cell line–derived neurotrophic factor (Peprotech), 1 mM dibutyryl cyclic-AMP sodium salt (Sigma-Aldrich), and 200 nM ascorbic acid (STEMCELL Technologies), making complete BrainPhysTM neural medium (Sundaramoorthy et al. ). ..

    Derivative Assay:

    Article Title: Inflammatory cytokines disrupt astrocyte exosomal HepaCAM-mediated protection against neuronal excitotoxicity in the SOD1G93A ALS model.
    Article Snippet: The MN pellets were gently resuspended with neuron plating medium containing DMEM, 10% of FBS (Sigma- Aldrich), and 1% Pen- Strep and plated on PDL + LN coated coverslips at 200,000 cells per well. .. MNs were incubated a 37°C for 12 hours, washed twice with warm neurobasal medium (Invitrogen), and cultured in 1 ml of MN medium: neurobasal medium supplemented with 2% B- 27 (Thermo Fisher Scientific), 1% of 100× GlutaMAX, hydrocortisone (0.2 μg/ml; Sigma- Aldrich), insulin (2.5 μg/ml; SigmaAldrich), human recombinant NT3 (10 ng/ml; ProspecBio), human recombinant glial cell line–derived neurotrophic factor (GDNF, 10 ng/ml; Peprotech), human recombinant brain- derived neurotrophic factor (BDNF, 10 ng/ml; R&D Systems), and human recombinant ciliary neurotrophic factor (25 ng/ml; Novus Biologicals). ..



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